Sino Biological, Inc. has announced that its gene synthesis and cell-free protein expression workflow was used in a recent study by Tencent AI for Life Sciences Lab published in Nature Communications. The study utilized Sino Biological's XPressMAX™ Cell-Free Protein Synthesis Kit to enable rapid experimental validation of AI-designed proteins, bridging the gap between computational predictions and functional outcomes.
Artificial intelligence has significantly accelerated protein amino acid sequence design, but translating these designs into functional proteins remains a key challenge. Protein activity, stability, folding, and expression are influenced by complex factors, often leading to discrepancies between in silico predictions and experimental results. To address this, the study introduced an Ontology Reinforcement Iteration (ORI) framework, integrating protein ontology with reinforcement learning from wet-lab feedback. Experimental data, including protein expression levels and functional activity, were continuously fed back into the model, enabling iterative optimization and improved design accuracy.
The researchers used Sino Biological's cell-free protein synthesis system for rapid protein expression and functional screening. Protein-coding sequences cloned into the kit's expression vector and added to the proprietary cell-free reaction system supported rapid design–build–test cycles. Using this workflow, the team engineered a lysozyme with over 100-fold higher activity than the natural enzyme, developed a thermostable chitinase retaining activity at 85°C, and successfully expressed bifunctional enzymes with improved performance compared with naturally occurring multifunctional enzymes.
The XPressMAX™ Cell-Free Protein Synthesis Kit offers ultra-fast synthesis with protein expression completed in as little as 3 hours, high screening efficiency optimized for VHH, scFv, and miniproteins, flexible template support for plasmid and linear DNA templates, disulfide bond-friendly expression without additional enhancers, cost-effective performance, and scalable supply for high-throughput and industrial applications.
Sino Biological is an international reagent supplier and CRO service provider specializing in recombinant protein production and antibody development. With the US-based Center for Bioprocessing (C4B) in Houston and SignalChem Biotech in Canada, the company delivers tailored solutions worldwide. Serving researchers in over 90 countries, Sino Biological maintains a stringent quality management system across all products. More information is available at www.sinobiological.com.
The original release can be viewed at www.newmediawire.com.
